Light rays striking the lens at different angles go through different points of the lens, not through the centre. 'Barrel distortion' is curvilinear distortion and is due to variations in magnification over the area of the lens. I know nothing about photography, but am eager to learn and would really appreciate any suggestions. Right now i am using a crappy Canon Powershot, but my lab is willing to upgrade to any camera required to make this work. How can I reduce this effect? Also, we would like to reduce vignetting as much as possible. In order to automate the analysis process there can be no fisheye. The problem I am currently facing is a fisheye effect (see attached photo). The current setup involves placing the plates on a light box, mounting a digital camera on a stand 1-3 feet above the plates, and quickly snapping pictures. However we would like to instead take pictures of our plates with a digital camera as it is faster and reduces glare. When the screens are finished we use to scan the plates into the computer (with a high end flatbed scanner) in order to automatically analyze them. Part of the project involves conducting high-throughput screens on rectangular agar plates. My project involves using yeast as a model to study human cancers. I am a PhD student at Columbia University and need some photography help.
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